{"id":36,"date":"2020-09-08T17:29:38","date_gmt":"2020-09-08T09:29:38","guid":{"rendered":"http:\/\/localhost\/PureCell\/?page_id=36"},"modified":"2022-06-24T14:01:27","modified_gmt":"2022-06-24T06:01:27","slug":"%e5%8d%95%e7%bb%86%e8%83%9e%e5%85%8d%e7%96%ab%e7%bb%84%e6%b5%8b%e5%ba%8f","status":"publish","type":"page","link":"https:\/\/www.chunxunbio.com\/?page_id=36","title":{"rendered":"\u5355\u7ec6\u80de\u514d\u75ab\u7ec4\u6d4b\u5e8f"},"content":{"rendered":"\n

\u5355\u7ec6\u80de\u514d\u75ab\u7ec4\u5e93\u6d4b\u5e8f<\/strong><\/h3>\n\n\n\n

          \u5355\u7ec6\u80de\u514d\u75ab\u7ec4\u5e93\u6d4b\u5e8f\u53c8\u79f0\u5355\u7ec6\u80deV(D)J\u6d4b\u5e8f\uff0c\u4ee5T\/B\u6dcb\u5df4\u7ec6\u80de\u4e3a\u7814\u7a76\u76ee\u6807\uff0c\u5229\u7528\u5fae\u6d41\u63a7\u82af\u7247\u5236\u5907\u5355\u7ec6\u80de\u4f53\u7cfb\uff0c\u5229\u7528PCR\u6280\u672f\u6269\u589eT\/B\u7ec6\u80de\u53d7\u4f53\uff08TCR\/BCR)\u7684\u57fa\u56e0\u8f6c\u5f55\u5b50\u5168\u5e8f\u5217\uff0c\u5728\u8fdb\u884c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u3002\u5206\u6790\u5f97\u5230TCR\/BCR\u591a\u6837\u6027\u5e8f\u5217\uff0c\u4ece\u800c\u83b7\u5f97\u673a\u4f53\u7684\u514d\u75ab\u7279\u5f81\u3002<\/p>\n\n\n\n

\u5355\u7ec6\u80de\u514d\u75ab\u7ec4\u6d4b\u5e8f\u6280\u672f\u6d41\u7a0b<\/strong><\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

\u6280\u672f\u53c2\u6570<\/strong><\/h3>\n\n\n\n

\u6837\u672c\u7c7b\u578b<\/strong>\uff1a\u65b0\u9c9c\u4eba\u6216\u5c0f\u9f20\u7ec6\u80de\u60ac\u6db2\u3001\u8840\u6db2\uff08\u5168\u8840\u3001PBMC\u7b49\uff09\u3001\u51bb\u5b58\u7ec6\u80de\u60ac\u6db2<\/p>\n\n\n\n

\u9001\u6837\u8981\u6c42<\/strong>\uff1a<\/p>\n\n\n\n

          \u7ec6\u80de\u5927\u5c0f\uff1a\u5c0f\u4e8e40\u03bcm\uff1b<\/span><\/p>\n\n\n\n

          \u6837\u54c1\u603b\u91cf\uff1a\u7ec6\u80de\u60ac\u6d6e\u6db2\uff0c\u4e00\u4e2a\u6837\u672c\u7ec6\u80de\u8d77\u59cb\u91cf\u7ea61×105<\/sup>\u4e2a\uff1b<\/span><\/p>\n\n\n\n

          \u8d28\u91cf\u8981\u6c42\uff1a\u7ec6\u80de\u6d3b\u6027\u5927\u4e8e80%\uff0c\u7406\u60f3\u6d53\u5ea6700~1200\u4e2a\/\u03bcL(500~2000\u4e2a\/\u03bcl)\uff1b<\/span><\/p>\n\n\n\n

\u5e94\u7528\u9886\u57df<\/strong><\/h2>\n\n\n\n

\u80bf\u7624\u514d\u75ab\u6cbb\u7597<\/strong>\uff1a<\/span><\/p>\n\n\n\n

          \u5355\u7ec6\u80de\u514d\u75ab\u5e93\u6d4b\u5e8f\u53ef\u4ee5\u6355\u6349\u80bf\u7624\u53d1\u751f\u65f6\u7684\u514d\u75ab\u5fae\u73af\u5883\u53d8\u5316\uff0c\u5bfb\u627e\u514d\u75ab\u6cbb\u7597\u7684\u9776\u70b9\uff0c\u4ece\u800c\u8f85\u52a9\u514d\u75ab\u6cbb\u7597\u66f4\u597d\u5730\u6297\u51fb\u80bf\u7624\u3002 <\/p>\n\n\n\n

\u514d\u75ab\u7cfb\u7edf\u75be\u75c5\u7814\u7a76<\/span><\/strong><\/p>\n\n\n\n

          \u89e3\u6790\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5\u7684\u53d1\u75c5\u673a\u5236\uff0c\u4e3a\u75be\u75c5\u7684\u8bca\u7597\u63d0\u4f9b\u4f9d\u636e\u3002<\/p>\n\n\n\n

\u79fb\u690d\u548c\u514d\u75ab\u91cd\u5efa<\/span><\/strong><\/p>\n\n\n\n

          \u5668\u5b98\u6216\u8005\u9aa8\u9ad3\u79fb\u690d\u65f6\uff0c\u7ecf\u5e38\u4f1a\u8bf1\u53d1\u5bbf\u4e3b\u7684\u6392\u65a5\u53cd\u5e94\uff0c\u4ece\u800c\u53d1\u751f\u6162\u6027\u79fb\u690d\u6297\u5bbf\u4e3b\u75c5\u3002\u5728\u75ab\u82d7\u63a5\u79cd\u524d\u540eBCR\/TCR VDJ\u514d\u75ab\u7ec4\u5e93\u7684\u5206\u6790\uff0c\u9884\u9632\u75be\u75c5\u53d1\u751f\u3002<\/p>\n\n\n\n

\u8f85\u52a9\u6297\u4f53\/\u75ab\u82d7\u7814\u7a76<\/span><\/strong><\/p>\n\n\n\n

          \u901a\u8fc7\u514d\u75ab\u7ec4\u5e93\u7814\u7a76\uff0c\u53ef\u4ee5\u68c0\u6d4b\u611f\u67d3\u7c7b\u75be\u75c5\u8fc7\u7a0b\u4e2d\u7684\u514d\u75ab\u52a8\u6001\u53d8\u5316\uff1b\u5728\u6297\u4f53\u5f00\u53d1\u65b9\u9762\uff0c\u53ef\u4ee5\u83b7\u5f97\u7279\u5f81\u6027\u7684BCR\uff08Ig\uff09\u5e8f\u5217\uff0c\u7f29\u77ed\u6297\u4f53\u5f00\u53d1\u7684\u6d41\u7a0b\uff1b\u9488\u5bf9\u67d0\u79cd\u75be\u75c5\u7528\u836f\u540e\u7684\u5916\u5468\u8840\u6837\u672c\u8fdb\u884c\u8bc4\u4f30\uff0c\u786e\u8ba4\u836f\u7269\u662f\u5426\u6fc0\u53d1\u514d\u75ab\u53cd\u5e94\u53ca\u5176\u529f\u6548\u3002<\/p>\n\n\n\n

<\/p>\n\n\n\n

\u5206\u6790\u5185\u5bb9<\/strong><\/h3>\n\n\n\n

\u6807\u51c6\u4fe1\u606f\u5206\u6790 \uff08\u9700\u63d0\u4f9b\u53c2\u8003\u57fa\u56e0\u5e8f\u5217\u3001\u53c2\u8003\u57fa\u56e0\u7ec4\u5e8f\u5217\u53ca\u57fa\u56e0\u6ce8\u91ca\u7ed3\u679c\uff09<\/p>\n\n\n\n

1\u3001\u6d4b\u5e8f\u6570\u636e\u7edf\u8ba1\u4e0e\u8bc4\u4f30<\/p>\n\n\n\n

2\u3001\u6bd4\u5bf9\u4e0e\u6ce8\u91ca\uff1a\u6837\u672c\u57fa\u672c\u4fe1\u606f\u7ed3\u679c\u7edf\u8ba1\u3001\u6837\u672ccontig\u6ce8\u91ca\u4fe1\u606f\u7ed3\u679c\u7edf\u8ba1\u3001\u6837\u672cconsensus\u6ce8\u91ca\u4fe1\u606f\u7ed3\u679c\u7edf\u8ba1<\/p>\n\n\n\n

3\u3001Clonotype\u5206\u578b<\/p>\n\n\n\n

4\u3001\u7279\u5f81\u5206\u6790\u3001CDR3\u7279\u5f81\u5206\u6790\u3001V\/J\u57fa\u56e0\u7279\u5f81\u5206\u6790\u3001V-J paired\u7279\u5f81\u5206\u6790<\/p>\n\n\n\n

5\u3001\u591a\u6837\u672c\u5206\u6790\uff1a\u6837\u672c\u95f4Clonotypes\u6bd4\u8f83\u3001Overlapping Clonotype\u805a\u7c7b\u5206\u6790\u3001Overlapping Clonotype\u5dee\u5f02\u5206\u6790<\/p>\n\n\n\n

<\/p>\n\n\n\n

\u6848\u4f8b\u89e3\u6790<\/strong><\/h3>\n\n\n\n

\u6587\u7ae0\uff1a<\/strong>The landscape of lung bronchoalveolar immune cells in COVID-19 revealed by single-cell RNA sequencing <\/p>\n\n\n\n

\u6742\u5fd7\uff1a<\/strong>Nature Medicine <\/p>\n\n\n\n

\u5f71\u54cd\u56e0\u5b50\uff1a<\/strong>36.130<\/p>\n\n\n\n

\u7b80\u4ecb\uff1a<\/span><\/strong><\/p>\n\n\n\n

          \u4f5c\u8005\u5bf9\u60a3\u8005\u80ba\u6ce1\u704c\u6d17\u6db2\uff08Balf\uff09\u8fdb\u884c\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u53ca\u5355\u7ec6\u80deTCR\u5206\u6790\uff0c\u6bd4\u8f83\u5206\u6790\u4e86\u91cd\u75c7\u60a3\u8005\u548c\u8f7b\u75c7\u60a3\u8005\u7684\u80ba\u90e8\u514d\u75ab\u5fae\u73af\u5883\u3002\u4e3a\u4e86\u8bc6\u522b\u65b0\u51a0\u611f\u67d3\u540e\u80ba\u90e8\u7684\u514d\u75ab\u5fae\u73af\u5883\uff0c\u4ee53\u4f8b\u6062\u590d\u671f\u7684\u8f7b\u75c7\u548c3\u4f8b\u91cd\u75c7\u60a3\u8005BALF\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u5171\u6536\u96c6\u523037820\u4e2a\u7ec6\u80de\u3002<\/p>\n\n\n\n

          \u7814\u7a76\u53d1\u73b0\u4e0e\u5065\u5eb7\u5bf9\u7167\u76f8\u6bd4\uff0cCOVID-19\u611f\u67d3\u7684\u60a3\u8005\u5b58\u5728\u66f4\u9ad8\u6bd4\u4f8b\u7684T\u548cNK\u7ec6\u80de\uff0c\u8f83\u4f4e\u6bd4\u4f8b\u7684\u4e0a\u76ae\u7ec6\u80de\u3002\u4e0e\u8f7b\u75c7\u60a3\u8005\u76f8\u6bd4\uff0c\u91cd\u75c7\u60a3\u8005\u542b\u6709\u66f4\u9ad8\u7684\u5de8\u566c\u7ec6\u80de\u6bd4\u4f8b\uff0c\u8f83\u4f4e\u6bd4\u4f8b\u7684T\u548cNK\u7ec6\u80de\uff1b\u5728\u91cd\u75c7COVID-19\u60a3\u8005\u4e2d\uff0c\u80ba\u5de8\u566c\u7ec6\u80de\u7fa4\u4f53\u7684\u5e73\u8861\u5931\u8c03\uff0c\u8868\u73b0\u4e3aGroup1 \u5355\u6838\u7ec6\u80de\u884d\u751f\u5de8\u566c\u7ec6\u80de\uff08FCN1 +\uff09\u3001Group2\u548c3 \u5de8\u566c\u7ec6\u80de\uff08SPP1 +\uff09\u663e\u7740\u589e\u52a0\uff0c\u800cGroup 2 lung AM\uff08FABP4+\uff09\u51e0\u4e4e\u5b8c\u5168\u6d88\u5931\uff1b\u4e0e\u5065\u5eb7\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u65b0\u51a0\u60a3\u8005\u4e2dNK\u548cT\u7ec6\u80de\u6bd4\u4f8b\u660e\u663e\u589e\u9ad8\u3002\u4e0e\u8f7b\u75c7\u60a3\u8005\u76f8\u6bd4\uff0c\u91cd\u75c7\u60a3\u8005CD8+T\u7ec6\u80de\u6bd4\u4f8b\u8f83\u4f4e\uff0c\u589e\u6b96\u7ec6\u80de\u6bd4\u4f8b\u8f83\u9ad8 \uff1b\u8f7b\u5ea6\u75be\u75c5\u7ec4\u7684\u603bT\u7ec6\u80de\u548cCD8+ T\u7ec6\u80de\u7684\u6269\u589e\u6c34\u5e73\u5747\u663e\u7740\u9ad8\u4e8e\u91cd\u75c7\u60a3\u8005\uff0c\u8868\u660e\u514b\u9686\u6269\u589e\u7684CD8+ T\u7ec6\u80de\u7684\u9ad8\u6548\u7684\u6548\u5e94\u5206\u5b50\u6c34\u5e73\uff1b \u6587\u7ae0\u4e3aCD8+ T\u7ec6\u80de\u53cd\u5e94\u53ef\u80fd\u662f\u6210\u529f\u63a7\u5236COVID-19\u60a3\u8005\u75c5\u6bd2\u7684\u5173\u952e\u63d0\u51fa\u4e86\u65b0\u7684\u8bc1\u636e\u3002<\/p>\n\n\n\n

<\/p>\n\n\n\n

\u7814\u7a76\u5c55\u793a\uff1a<\/strong><\/h3>\n\n\n\n

          \u4f5c\u8005\u5229\u7528\u5355\u7ec6\u80deTCR-seq\u6765\u8bc4\u4f30\u60a3\u8005BALF\u4e2d\u514b\u9686\u6269\u589e\u7684\u72b6\u6001\u3002\u68c0\u67e5\u4e86COVID-19\u8f7b\u75c7\u548c\u91cd\u75c7\u60a3\u8005\u4e4b\u95f4\u514b\u9686\u6269\u589e\u7684\u6bd4\u4f8b\uff0c\u53d1\u73b0\u8f7b\u5ea6\u75be\u75c5\u7ec4\u7684\u603bT\u7ec6\u80de\u548cCD8+ T\u7ec6\u80de\u7684\u6269\u589e\u6c34\u5e73\u5747\u663e\u7740\u9ad8\u4e8e\u91cd\u75c7\u60a3\u8005\uff08\u56fe b\u548c c\uff09\uff1b\u4e3a\u4e86\u786e\u8ba4\u6269\u589e\u7684T\u7ec6\u80de\u514b\u9686\u7684\u529f\u80fd\u72b6\u6001\uff0c\u7814\u7a76\u56e2\u961f\u5728\u6269\u589e\u7684CD8+ T\u7ec6\u80de\u4e0e\u672a\u6269\u589e\u7684\u7ec6\u80de\u4e4b\u95f4\u8fdb\u884c\u4e86GEX\u5206\u6790\uff0c\u53d1\u73b0\u4fe1\u53f7\u548c\u7ec4\u7ec7\u56fa\u6709\u57fa\u56e0\uff08\u5305\u62ecXCL1\u3001XCL2\u3001ZNF683\u3001HOPX\u3001CXCR6\u548cITGAE\u7b49\uff09\u7684\u8868\u8fbe\u589e\u52a0\u3002\u8fdb\u4e00\u6b65\u652f\u6301\u4e86\u90a3\u4e9b\u6269\u589e\u7684CD8+ T\u7ec6\u80de\u7684\u6548\u5e94\u5206\u5b50\u529f\u80fd\uff08\u56fe e\uff09\u3002<\/span>\"\"<\/p>\n\n\n\n

\u56fe  SARS-COV-2\u611f\u67d3\u60a3\u8005BALF\u4e2dT\u7ec6\u80de\u514b\u9686\u6269\u589e\u7684\u5206\u6790<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5355\u7ec6\u80de\u514d\u75ab\u7ec4\u5e93\u6d4b\u5e8f           \u5355\u7ec6\u80de\u514d\u75ab […]<\/p>\n","protected":false},"author":1,"featured_media":1006,"parent":34,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/pages\/36"}],"collection":[{"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=36"}],"version-history":[{"count":15,"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/pages\/36\/revisions"}],"predecessor-version":[{"id":986,"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/pages\/36\/revisions\/986"}],"up":[{"embeddable":true,"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/pages\/34"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=\/wp\/v2\/media\/1006"}],"wp:attachment":[{"href":"https:\/\/www.chunxunbio.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=36"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}